![]() ![]() You can customize the symbols, colors, axis labels, etc. ![]() The graph Prism makes automatically is fairly complete. So a concentration of 1 micromolar (10 -6 Molar) would be entered as -6. Why are X values negative? Because in this example, the X values are the logarithm of concentrations expressed in molar. Note that three of the four unknowns are labeled, so you can later match up the results with the labels. The goal of this analysis is to interpolate the corresponding X values (concentrations) for these unknowns. These have a Y values that you measured, but no X. The first seven rows contain the standard curve, in duplicate. You can move the floating note out of the way, or minimize it. The sample data may be partly covered by a floating note explaining how to fit the data (for people who are not reading this help page). Interpolate unknowns from sigmoidal curve. From the Welcome or New Table dialog, choose to create an XY data table, and select the sample data set: RIA or ELISA.
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